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ec2 3 dcas9 mxi1 sgrna dcas9 mxi1 expression vector  (Addgene inc)


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    Structured Review

    Addgene inc ec2 3 dcas9 mxi1 sgrna dcas9 mxi1 expression vector
    Ec2 3 Dcas9 Mxi1 Sgrna Dcas9 Mxi1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+expression+vector/pcDNA3%2E1+Orai3+(Plasmid+%2316370)/pmc12645569-47-1-5
    Average 93 stars, based on 5 article reviews
    ec2 3 dcas9 mxi1 sgrna dcas9 mxi1 expression vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
    Article Snippet: Recombinant DNA reagent , pSplit2-NO-PRPF6 (plasmid) , , RRID: Addgene_51740 , pSplit2 expression vector. .. Recombinant DNA reagent , pcDNA3.1 SAFB (plasmid) , , RRID: Addgene_32742 , pcDNA3.1 expression vector. .. Recombinant DNA reagent , GUM BUB3 (plasmid) , , RRID: Addgene_84029 , expression vector.

    Plasmid Preparation:

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
    Article Snippet: Recombinant DNA reagent , pSplit2-NO-PRPF6 (plasmid) , , RRID: Addgene_51740 , pSplit2 expression vector. .. Recombinant DNA reagent , pcDNA3.1 SAFB (plasmid) , , RRID: Addgene_32742 , pcDNA3.1 expression vector. .. Recombinant DNA reagent , GUM BUB3 (plasmid) , , RRID: Addgene_84029 , expression vector.

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly.
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Article Title: Microplastic in northern anchovies (Engraulis mordax) and common murres (Uria aalge) from the Monterey Bay, California USA - Insights into prevalence, composition, and estrogenic activity.
    Article Snippet: .. Briefly, human embryonic kidney cells (HEK293) were co-transfected with the human estrogen receptor 1 gene (hESR1) in a pcDNA3.1+ expression vector (www.cdna.org), pCMX- β-galactosidase (β-gal) and pGL2-3xERE luciferase reporter plasmids (Addgene plasmid 11,354)(Hall and McDonnell, 1999). .. After 24 h cells were treated, in triplicate, with an endogenous estrogen 17β-estradiol (E2: 10− 12-10− 7 M; Steraloids, Newport, RI), BPA (10− 5 M; Sigma-Aldrich, St. Louis, MO), a vehicle treatment of 0.1% DMSO, 0.1% EtOH control, or microparticle leachate.

    Article Title: Acquired resistance to vemurafenib restrains thyroid cancer stem cell self-renewal by suppressing STAT3 activation.
    Article Snippet: Vemurafenib (PLX4032) is a B-Raf kinase-specific inhibitor that has been approved for treating BRAF-mutated melanoma but is ineffective in treating thyroid cancer.. Cancer stem cells (CSCs) play an important role in drug resistance (DR).. Our present study aims to determine the status of CSCs in thyroid cancer cells that have acquired adaptive drug resistance to PLX4032.

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Expressing:

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
    Article Snippet: Recombinant DNA reagent , pSplit2-NO-PRPF6 (plasmid) , , RRID: Addgene_51740 , pSplit2 expression vector. .. Recombinant DNA reagent , pcDNA3.1 SAFB (plasmid) , , RRID: Addgene_32742 , pcDNA3.1 expression vector. .. Recombinant DNA reagent , GUM BUB3 (plasmid) , , RRID: Addgene_84029 , expression vector.

    Article Title: Pharmacological Activation of SIRT3 Modulates the Response of Cancer Cells to Acidic pH.
    Article Snippet: Bafilomycin A1 (B1793, Merck, St. Louis, MO, USA) was dissolved in DMSO and added to a final concentration of 100 nM for the times indicated. .. MDA-MB-231 cells were stably transfected with a pcDNA3.1 expression vector encoding for human SIRT3-Flag (Addgene Inc., Cambridge, MA, USA). .. Stable clones were generated by delivering plasmid DNA constructs into cells using TurboFectin 8.0 (Origene Technologies, Rockville, MD, USA).

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly.
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Article Title: Microplastic in northern anchovies (Engraulis mordax) and common murres (Uria aalge) from the Monterey Bay, California USA - Insights into prevalence, composition, and estrogenic activity.
    Article Snippet: .. Briefly, human embryonic kidney cells (HEK293) were co-transfected with the human estrogen receptor 1 gene (hESR1) in a pcDNA3.1+ expression vector (www.cdna.org), pCMX- β-galactosidase (β-gal) and pGL2-3xERE luciferase reporter plasmids (Addgene plasmid 11,354)(Hall and McDonnell, 1999). .. After 24 h cells were treated, in triplicate, with an endogenous estrogen 17β-estradiol (E2: 10− 12-10− 7 M; Steraloids, Newport, RI), BPA (10− 5 M; Sigma-Aldrich, St. Louis, MO), a vehicle treatment of 0.1% DMSO, 0.1% EtOH control, or microparticle leachate.

    Article Title: Acquired resistance to vemurafenib restrains thyroid cancer stem cell self-renewal by suppressing STAT3 activation.
    Article Snippet: Vemurafenib (PLX4032) is a B-Raf kinase-specific inhibitor that has been approved for treating BRAF-mutated melanoma but is ineffective in treating thyroid cancer.. Cancer stem cells (CSCs) play an important role in drug resistance (DR).. Our present study aims to determine the status of CSCs in thyroid cancer cells that have acquired adaptive drug resistance to PLX4032.

    Article Title: Exosomal LINC00853 promotes progression of gastric cancer via the MAP17/PDZK1/AKT signaling pathway
    Article Snippet: .. LINC00853 was used to construct an overexpression vector using the pcDNA3.1(+) expression vector purchased from Addgene using the following cloning primer sequences: LINC00853_NheI_F (acccaagctggctagcGCCGCGCCTGAAGCTCAACT) and LINC00853_Xbal_R (aaacgggccctagaTGACACATGCAGAAATACTAT). .. The completed vector was transfected into AGS cells with Lipofectamine 2000 (Invitrogen) for 24 h with 1 μg of pcDNA3.1-LINC00853 to induce overexpression.

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Article Title: Long non-coding RNA LUCAT1 regulates the RAS pathway to promote the proliferation and invasion of malignant glioma cells through ABCB1.
    Article Snippet: Long non-coding RNAs (lncRNAs) are closely related to the occurrence and development of tumors and have gradually become a hot topic in the field of glioma research in recent years.. In this study, the role of lung cancer associated transcript 1 (lncRNA LUCAT 1) in glioma occurrence and development, as well as its possible regulatory mechanism, was explored.. We utilized the gene chip technology in the preliminary experiment, and based on the experiment results, selected LUCAT1(NONHSAT102745), which was significantly upregulated in glioma, and ATP-binding cassette Subfamily B member l (ABCB1), which was significantly down-regulated in coexpression analysis, for study.

    Multiple Displacement Amplification:

    Article Title: Pharmacological Activation of SIRT3 Modulates the Response of Cancer Cells to Acidic pH.
    Article Snippet: Bafilomycin A1 (B1793, Merck, St. Louis, MO, USA) was dissolved in DMSO and added to a final concentration of 100 nM for the times indicated. .. MDA-MB-231 cells were stably transfected with a pcDNA3.1 expression vector encoding for human SIRT3-Flag (Addgene Inc., Cambridge, MA, USA). .. Stable clones were generated by delivering plasmid DNA constructs into cells using TurboFectin 8.0 (Origene Technologies, Rockville, MD, USA).

    Stable Transfection:

    Article Title: Pharmacological Activation of SIRT3 Modulates the Response of Cancer Cells to Acidic pH.
    Article Snippet: Bafilomycin A1 (B1793, Merck, St. Louis, MO, USA) was dissolved in DMSO and added to a final concentration of 100 nM for the times indicated. .. MDA-MB-231 cells were stably transfected with a pcDNA3.1 expression vector encoding for human SIRT3-Flag (Addgene Inc., Cambridge, MA, USA). .. Stable clones were generated by delivering plasmid DNA constructs into cells using TurboFectin 8.0 (Origene Technologies, Rockville, MD, USA).

    Transfection:

    Article Title: Pharmacological Activation of SIRT3 Modulates the Response of Cancer Cells to Acidic pH.
    Article Snippet: Bafilomycin A1 (B1793, Merck, St. Louis, MO, USA) was dissolved in DMSO and added to a final concentration of 100 nM for the times indicated. .. MDA-MB-231 cells were stably transfected with a pcDNA3.1 expression vector encoding for human SIRT3-Flag (Addgene Inc., Cambridge, MA, USA). .. Stable clones were generated by delivering plasmid DNA constructs into cells using TurboFectin 8.0 (Origene Technologies, Rockville, MD, USA).

    Amplification:

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly.
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Clone Assay:

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly.
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Article Title: Vibrio cholerae cytolysin induces pro-inflammatory and death signals through novel TLR assembly
    Article Snippet: RNA isolated from the untreated RAW264.7 macrophage cells was used to generate the cDNA using Superscript III reverse transcriptase kit (Invitrogen). .. Nucleotide sequences for TLR2, TLR4, TLR6, CD36, MD-2, and CD14 were amplified from the cDNA preparation and cloned into the pcDNA3.1(+) expression vector. pcDNA3.1-TLR1 plasmid was from Addgene (Plasmid #13080). ..

    Luciferase:

    Article Title: Microplastic in northern anchovies (Engraulis mordax) and common murres (Uria aalge) from the Monterey Bay, California USA - Insights into prevalence, composition, and estrogenic activity.
    Article Snippet: .. Briefly, human embryonic kidney cells (HEK293) were co-transfected with the human estrogen receptor 1 gene (hESR1) in a pcDNA3.1+ expression vector (www.cdna.org), pCMX- β-galactosidase (β-gal) and pGL2-3xERE luciferase reporter plasmids (Addgene plasmid 11,354)(Hall and McDonnell, 1999). .. After 24 h cells were treated, in triplicate, with an endogenous estrogen 17β-estradiol (E2: 10− 12-10− 7 M; Steraloids, Newport, RI), BPA (10− 5 M; Sigma-Aldrich, St. Louis, MO), a vehicle treatment of 0.1% DMSO, 0.1% EtOH control, or microparticle leachate.

    Construct:

    Article Title: Exosomal LINC00853 promotes progression of gastric cancer via the MAP17/PDZK1/AKT signaling pathway
    Article Snippet: .. LINC00853 was used to construct an overexpression vector using the pcDNA3.1(+) expression vector purchased from Addgene using the following cloning primer sequences: LINC00853_NheI_F (acccaagctggctagcGCCGCGCCTGAAGCTCAACT) and LINC00853_Xbal_R (aaacgggccctagaTGACACATGCAGAAATACTAT). .. The completed vector was transfected into AGS cells with Lipofectamine 2000 (Invitrogen) for 24 h with 1 μg of pcDNA3.1-LINC00853 to induce overexpression.

    Over Expression:

    Article Title: Exosomal LINC00853 promotes progression of gastric cancer via the MAP17/PDZK1/AKT signaling pathway
    Article Snippet: .. LINC00853 was used to construct an overexpression vector using the pcDNA3.1(+) expression vector purchased from Addgene using the following cloning primer sequences: LINC00853_NheI_F (acccaagctggctagcGCCGCGCCTGAAGCTCAACT) and LINC00853_Xbal_R (aaacgggccctagaTGACACATGCAGAAATACTAT). .. The completed vector was transfected into AGS cells with Lipofectamine 2000 (Invitrogen) for 24 h with 1 μg of pcDNA3.1-LINC00853 to induce overexpression.

    Cloning:

    Article Title: Exosomal LINC00853 promotes progression of gastric cancer via the MAP17/PDZK1/AKT signaling pathway
    Article Snippet: .. LINC00853 was used to construct an overexpression vector using the pcDNA3.1(+) expression vector purchased from Addgene using the following cloning primer sequences: LINC00853_NheI_F (acccaagctggctagcGCCGCGCCTGAAGCTCAACT) and LINC00853_Xbal_R (aaacgggccctagaTGACACATGCAGAAATACTAT). .. The completed vector was transfected into AGS cells with Lipofectamine 2000 (Invitrogen) for 24 h with 1 μg of pcDNA3.1-LINC00853 to induce overexpression.



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